Yoshino is a new character introduced by Metal Slug Attack.
Like Dragunov (another Ptolemaic Army commando), she possesses an Amazonian build - they're both tall and muscular; although she instead has dark black hair and golden eyes (whereas Dragunov is a blond). She wears a variant of the Ptolemaic Special Forces jumpsuit and face mask alongside a red scarf.
Trained in the art of ninjutsu, she can move so quickly as to appear to teleport - she normally shuffles slowly under the weight of her immense hammer, but she's able to leap huge distances with it, and can also move in the blink of an eye when using smoke bombs. She is also capable of creating clones of herself to damage enemies.
Her MSA infobox states that she is able to manipulate prototype weapons better then anyone else (most likely due to her superhuman strength). She gets along well with Dragunov and Caroline, two other members of the Ptolemaic Army.
Yoshino is on duty to train her fellow subordinates in the Extra Ops "Destroy the Tower". While the soldiers are on their break, Amadeus Army deploys Toschka Dalanue. The tower kills her soldiers. Angered, she appears and hammers away the bottom three segments of the tower. After destroying the Toschka Dalanue, Amadeus robots surround her and self-destruct in an attempt to kill her. Fortunately, Dragunov arrived and saved her.
She later appears in the Extra Ops "Flying Ace", where she is fighting several Tar Men and a Dark Monoeye. She has difficulty killing the Tar Men until Caroline appears and helps her kill them. When the Dark Monoeye attempts to defend itself with monoliths, Caroline counterattacks with her ShootingRay. This allows Yoshino to move in and kill the Dark Monoeye.
In "No Limit", she saves Caroline before Destrade is able to kill her, stating that she will have Dragunov discipline her for disobeying orders.
In "Eternal Way", after Caroline and Towa destroy the Sea Satan+, Yoshino approaches Caroline and drags her away for disciplining for disobeying orders again.
Sometime later in "Growing Ninja", Yoshino decides to take a disciple named Sho and teaches him the way of the ninja. When Schwarz Metzelei appears with a Flying Shelt, Yoshino tasks him with tackling the two opponents alone, disappearing from sight and supposedly abandoning him. Sho manages to destroy the Flying Shelt and drives Schwarz off with his "Air Rend" skill. Yoshino congratulates him for his victory, but slaps him across the face to "knock some sense" back into him and for failing to notice her presence, telling him that he is still learning.
Yoshino appears in Another Story in several settings.
In Scrap Life, she and Dragunov go on a mission to acquire the Big Shiee, though when Caroline comes along despite being told not to, has Dragunov take care of her for the duration of the mission. Yoshino holds off Allen O'Neil when he manages to wound Dragunov and successfully escapes after stalling him long enough.
In Ωmegalpha, Yoshino is informed of an attack on their base by unknown mercenaries. She discovers the Red Goblin and quickly deduces that he is here for Ptolemaios since the base is not that damaged. She battles him, only to be defeated and warned not to intervene again. Yoshino tries to move but is unable to, realizing that the wound he inflicted on her is deeper then she thought.
In "The Dream of the Prodigious Mechanic", Yoshino helps Dragunov look for Caroline after she runs off, despite not having completely recovered from her battle with Red Goblin. Yoshino finds Caroline and calmly tells her what the Ptolemaic Army does to survive, and assures her that she will see Crablops. The twin tanks Shoe & Karn ambush them, but they are able to destroy them and continue on. Yoshino later teases Dragunov about how "some people" never change when Caroline begs for a monkey wrench.
In "Cloak of Darkness", Yoshino can be seen conversing with Dragunov, who later tasks her with finding the commander-in-chief (Ptolemaios) due to being a complete mystery for the army. In the secret ending, she also learns about Shizuka from Caroline, shocking her.
In "The Legend of the Samurai", Yoshino arrives on a deserted island to find the fabled samurai living on the island and acquire her fro the Ptolemaic Army. She meets Red and his group again. When she finds the samurai Jin, she challenges her to a battle. Both take heavy wounds, and Yoshino is dragged away by her soldiers despite her protests. When the Sand Marine gets destroyed, she orders a retreat.
In "Everlasting Summer Vacation", Yoshino is taking a break off with Dragunov at a local resort. She and most of the other army women on vacation overhear a Martian awakening that will allow Rootmars to give one wish, and begin to capture Martians. Abigail and Trevor later confront the two to free their Martians. They are defeated, but Abigail manages to surprise Yoshino with an attack, causing her to drop in the water. Despite pleas from Dragunov to stay afloat, Yoshino allows herself to sink, as a way to "train" herself.
When Caroline expresses her desire to obtain the Sergeant Drill, Yoshino and Dragunov accompany her into the base. Upon encountering Allen and Destrade, the two sides fight each other to settle unfinished matters. Destrade attempts to grab Caroline, but Yoshino stops her. She then tells the mechanic to support Dragunov, and eventually defeats Destrade in battle to rejoin her fellow comrades.
- In Metal Slug Attack, she goes into battle wearing a face mask. If an attack manages to knock her back, this will be removed and she'll continue without it.
- In her Everlasting Summer outfit, Yoshino carries three kunai on her, though she does not use it in battle.
- She and Dragunov is believed to be very strong because they can carry their weapons with only one hand.
- She is available for purchase at the United Shop in the 3rd United Front event.