Here's the list of first introduction in Metal Slug series by faction.
Regular Army[]
- First male protagonist: Marco Rossi
- First 2P male appears as a selectable character: Tarma Roving (Debut in Metal Slug 2/Metal Slug X)
- First female protagonists Eri Kasamoto and Fio Germi (Debut in Metal Slug 2/Metal Slug X)
- First SNK protagonists to appear in Metal Slug games: Ralf Jones, Clark Still, Leona Heidern, Heidern, and Whip
- First Drill Instructor to have an in-game sprite: Sophia (Debut in Metal Slug Attack
Rebel Army[]
- First main antagonist that appears as a boss: Donald Morden
- First major antagonist appears as a sub-boss Allen O'Neil
- First female Rebel Army member: Abigail
- First experimental soldier: Vita
- First scientist to work for the Rebel army: Navy
- First character to have a personal vehicle: Rapid
- First conscripted infantry: Huracan